报告题目：Functional Dynamics of Cryptochrome Studied by Single-Molecule Techniques and SAXS

报告人：谭砚文，复旦大学物理系教授，博士生导师

报告时间：2021年3月15日 上午9:30

报告地点：瘦西湖校区新教学楼S405

报告摘要：Single-molecule fluorescent spectroscopy can provide unique perspectives which complement other bulk biophysical and biochemical approaches. We use single-molecule techniques to illuminate the working mechanism of a light-sensitive protein, cryptochrome, at different stages of a signaling transduction. Cryptochrome is a kind of signaling proteins that is known to retrain circadian clock and involves in the magnetic sensing capabilities of animals. Using smFRET (single-molecule Föster-type resonance energy transfer), we reveal how blue light would initiate in a conformational change in an algae cryptochrome and this conformational change is believed to be functionally important. While conformational changes are usually speculated as the cause of activation of signaling function in various receptor proteins, smFRET can afford direct evidence to these hypotheses.

Combining the results of smFRET, tertiary structure prediction, SAXS, and molecular dynamics, we were able to reconstruct the molecular details of cryptochromes’ light-induced conformational change, dimerization, and interactions with their downstream signaling partners.

报告人简介：谭砚文，复旦大学物理系教授，博士生导师。1998年六月自台湾大学物理系毕业后，于台湾中央研究院原子分子研究所范文祥实验室从事发光共轭高分子研究并于2000年六月取得台湾大学物理硕士学位。同年九月至美国哥伦比亚大学诺奖得主Horst Störmer实验室攻读博士学位。2006年底博士毕业，加入杨皓实验室，先后在加州伯克利大学（2007年二月至2009年六月）与普林斯顿化学系（2009年七月至2010年八月），以单分子荧光光谱学方法，研究蛋白质分子构象动力学相关的生物物理问题。2010年九月加入复旦大学物理系。迄今发表SCI论文十数篇，其中包含Nature一篇以及Phys. Rev. Lett. 5篇，JACS，Nature Communications各一篇。